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6 Steps to Pregnancy Test Kit Use for Accurate Results


When there is a suspicion of pregnancy, the most reliable way to confirm it is by using a pregnancy test kit. Home pregnancy tests are convenient and straightforward to perform. However, it is vital to understand the mechanism behind how they work and conduct a proper pregnancy test kit use to obtain precise and accurate results.

Incorrect pregnancy test results might cause anxiety, misunderstanding, or delay in seeking medical assistance. False-positive results can be a result of certain medical conditions or medications, while false-negative results can occur if the test is taken too early or not used correctly.

This content will guide you through the process of using a home pregnancy test kit correctly, ensuring that you get accurate results. Go through the complete content so that you don’t miss any vital information about the test kit’s use and results.

How does a pregnancy test work?

Urine contains a pregnancy hormone called human chorionic gonadotropin (hCG), which forms the basis for the functioning of home pregnancy tests. Your body starts producing this hormone once a fertilised egg settles in your uterus.

Your body experiences a rapid increase in the amount of hCG during early pregnancy, and that’s the reason why home tests can detect it.

Proper pregnancy test kit use for accurate results

Proper pregnancy test kit use is crucial for obtaining accurate results. You should use the following steps:

1. Check the expiration date: Make sure the pregnancy test kit is not expired. Using an expired test kit could affect the accuracy of the results.

2. Collect urine: Collect your first-morning urine or hold your urine for at least four hours before collecting a sample. Collect the pee in a dry, clean container. Keep your hands away from the container’s interior.

3. Read the instructions: Read the instructions that come with the test kit carefully. Make sure you understand how to use the kit, how long to wait before reading the results, and what a positive or negative result looks like.

4. Test the urine: Place the test stick or dip the test strip into the urine for the specified amount of time, usually a few seconds. Ensure that you fully immerse the stick or strip in the urine.

5. Wait for the results: Follow the instructions for how long to wait before reading the results. Typically, results can be read within a few minutes.

6. Interpret the results: Look for the appearance of lines, colors, or symbols on the test stick or strip. A positive result will usually show a line, color, or symbol indicating the presence of the pregnancy hormone hCG. A negative result will usually show a single line or a negative symbol indicating the absence of hCG.

What are the common cases of inaccurate pregnancy test results?

There are several factors that can affect the accuracy of a pregnancy test result, including:

Certain medications or medical conditions: Fertility treatments, urinary tract infections (UTIs), recent miscarriage or abortion, ectopic pregnancy, and some cancers might influence hCG levels and cause false-positive or false-negative results.

Taking the test too early: Pregnancy tests detect hCG in urine. However, the amount of hCG in your body may not be detectable in the early stages of pregnancy, especially if you take the test before the first day of your missed period.

Drinking too much water: Drinking too much water or other fluids before taking the test may dilute the amount of hCG in your urine, potentially leading to a false-negative result.

Not following instructions carefully: Each pregnancy test kit comes with specific instructions on how to use it properly. Failure to follow the instructions, such as not collecting enough urine or not waiting long enough to read the results, can lead to inaccurate results.

When you can do a pregnancy test?

A woman with normal menstrual cycles can use a pregnancy test kit for accurate results on her first missed period. At this time, the hCG levels are typically high enough for the kit to detect a pregnancy. However, it’s essential to note that some women may not experience a missed period until several days or even weeks after conception. In such cases, it’s advisable to wait a few more days for the proper pregnancy test kit used to obtain accurate results.

Women with irregular menstrual cycles may have trouble determining ovulation and the best time to take a pregnancy test. In such cases, it’s best to wait for at least ten days after ovulation before testing.

This is because it takes some time for the fertilized egg to implant in the uterus and for hCG levels to rise to detectable levels. If a woman takes the test too early, she may get a false negative result.

Pregnancy test results

Pregnancy test results can be positive or negative. A positive result indicates that you are pregnant, while a negative result means you are not pregnant or took the test too early.

Positive pregnancy test


A positive pregnancy test simply indicates that you are pregnant. If you get a positive result, it is advisable to consult your doctor or a healthcare professional for confirmation and further advice.

Negative result


A negative result indicates you are either not pregnant or took the test too early. If you get a negative result and still have symptoms of pregnancy, you may need to retest in a few days or consult your doctor.

Must-know facts for better pregnancy test kit use

Human chorionic gonadotropin (hCG)

Human Chorionic Gonadotropin (hCG) is a hormone produced by the cells that form the placenta after a fertilized egg implants in the uterus. hCG is responsible for maintaining the corpus luteum, which in turn produces progesterone, essential for the development of the fetus.

Amount of hCG

It is important to note that the amount of hCG in the body can vary widely between individuals and pregnancies. Some women may have higher levels of hCG than others, while some pregnancies may have lower levels of hCG than expected. The hCG can affect the accuracy of the home pregnancy test, especially if you take the test too early.

The amount of hCG in the body increases rapidly during early pregnancy, typically doubling every two to three days. These tests help to detect even small amounts of hCG in the urine.

Home pregnancy testing kits

Home pregnancy tests are simple to use and easily available. They detect hCG in urine and confirm pregnancy.

Test too early

Pregnancy tests may not detect hCG in your urine if you do it too early. It is best to wait until the first day of your missed period or at least ten days after your ovulation before testing.

If you are not sure you want to be pregnant, it is advisable to seek professional advice and discuss your options. Many resources are available to help you make an informed decision, including counselling, support groups, and healthcare professionals.


Proper pregnancy test kit use can provide a reliable and convenient means of confirming pregnancy. You can get accurate results by following the instructions and using the testing kit correctly. If you still have doubts, visit Diwya Vatsalya Mamta IVF Centre in Patna, where our gynecologists provide free advice.